Characterization of translationally controlled tumour protein from the sea anemone Anemonia viridis and transcriptome wide identification of cnidarian homologues

Salvatore Costa, Marilena Vita Di Natale, Maria Antonietta Ragusa, Calogera Monastero, Girolama Biondo, Aldo Nicosia, Tiziana Masullo, Carmelo Bennici, Marilena Di Natale, Calogera Monastero, Marcello Tagliavia, Angela Cuttitta

Research output: Contribution to journalArticle

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Abstract

Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses.
Original languageEnglish
Pages (from-to)00-00
Number of pages21
JournalGenes
Volume9
Publication statusPublished - 2018

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Sea Anemones
Cnidaria
Transcriptome
Mediterranean Sea
Hydra
Messenger RNA
Molecular Evolution
Heavy Metals
Introns
translationally-controlled 1 tumor protein
Exons
Genome
Temperature
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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Characterization of translationally controlled tumour protein from the sea anemone Anemonia viridis and transcriptome wide identification of cnidarian homologues. / Costa, Salvatore; Di Natale, Marilena Vita; Ragusa, Maria Antonietta; Monastero, Calogera; Biondo, Girolama; Nicosia, Aldo; Masullo, Tiziana; Bennici, Carmelo; Di Natale, Marilena; Monastero, Calogera; Tagliavia, Marcello; Cuttitta, Angela.

In: Genes, Vol. 9, 2018, p. 00-00.

Research output: Contribution to journalArticle

Costa, Salvatore ; Di Natale, Marilena Vita ; Ragusa, Maria Antonietta ; Monastero, Calogera ; Biondo, Girolama ; Nicosia, Aldo ; Masullo, Tiziana ; Bennici, Carmelo ; Di Natale, Marilena ; Monastero, Calogera ; Tagliavia, Marcello ; Cuttitta, Angela. / Characterization of translationally controlled tumour protein from the sea anemone Anemonia viridis and transcriptome wide identification of cnidarian homologues. In: Genes. 2018 ; Vol. 9. pp. 00-00.
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abstract = "Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses.",
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AU - Costa, Salvatore

AU - Di Natale, Marilena Vita

AU - Ragusa, Maria Antonietta

AU - Monastero, Calogera

AU - Biondo, Girolama

AU - Nicosia, Aldo

AU - Masullo, Tiziana

AU - Bennici, Carmelo

AU - Di Natale, Marilena

AU - Monastero, Calogera

AU - Tagliavia, Marcello

AU - Cuttitta, Angela

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AB - Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses.

KW - Cnidarians

KW - Comparative genomics

KW - Gene expression

KW - Genetics

KW - Genetics (clinical)

KW - Homology modelling

KW - TCTPs

KW - Transcriptome wide analysis

KW - Translationally controlled tumour protein

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