Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures

Giorgio Mannina, María Eugenia Suárez-Ojeda, Gabriela Montiel-Jarillo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH 4 -Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
Original languageEnglish
Pages (from-to)361-369
Number of pages9
JournalBioresource Technology
Volume282
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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