Bioactive Polyphenols from Pomegranate Juice Reduce 5-Fluorouracil-Induced Intestinal Mucositis in Intestinal Epithelial Cells

Stella Maria Cascioferro, Giulia Auriemma, Giacomo Pepe, Emanuela Salviati, Shara Francesca Rapa, Alessia Bertamino, Stefania Marzocco, Pietro Campiglia, Giuseppina Autore, Andrea Quaroni

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Intestinal epithelial cells (IECs) play a pivotal role in maintaining intestinal homeostasis.Dierent noxious agents, among them also anticancer therapies, can impair intestinal epithelialintegrity triggering inflammation and oxidative stress. A frequent complication of chemotherapy isgastrointestinal mucositis, strongly influencing the eectiveness of therapy, increasing healthcare costs,and impairing patients’ quality of life. Dierent strategies are used to treat gastrointestinal mucositis,including products from natural sources. Our study focused on the eect of pomegranate (Punicagranatum L.) juice extract on IEC-6 cells, both during inflammatory conditions and following treatmentwith 5-fluorouracil (5-FU). The polyphenolic profile of pomegranate juice was characterized in detail byOnline Comprehensive two dimensional Liquid Chromatography-Mass Spectrometry. The evaluationof pomegranate juice extract in IEC-6 indicates a significant inhibition in proinflammatory factors,such as cytokines release, cyclooxygenase-2 and inducible nitric oxide synthase expression, andnitrotyrosine formation. Pomegranate also inhibited oxidative stress and adhesion protein expression.In 5-FU-treated IEC-6, pomegranate also inhibited both inflammatory and oxidative stress parametersand apoptosis. It promoted wound repair and tight junction expression. These results suggesta potential use of pomegranate as an adjuvant in the treatment of intestinal inflammatory andoxidative stress states, which also occur during chemotherapy-induced mucositis.
Original languageEnglish
Number of pages20
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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