[automatically translated] Numerous studies have found that fluid in the coelomic epithelium cells echinoderms are direct activities towards foreign agents. In particular the celomociti play a repertoire of immune functions including phagocytosis, cytotoxicity, the antibacterial activity, the formation of capsules and the rejection of transplants. In the present work we studied the cytotoxic activity of celomociti of Holothuria tubulosa (Oloturoideo) not separated and we used mammalian cells as targets. The celomociti taken from the coelomic epithelium fluid lyse the rabbit or sheep erythrocytes and tumor cells of the human eritromieloide K562. Also, the supernatant of the lysate of celomociti (CLS) showed lytic activity against the same target cell. The analysis of the CLS through an "overlay" of sheep erythrocytes on a polyacrilamide gel? in the absence of SDS, he has revealed the presence of at least two bands with different lytic activity and electrophoretic mobility. The bands lytic eluted and analyzed by SDS-PAGE showed a molecular size of 79 and 44 kDa. These data prelude to further research for the identification of the cell types responsible for the activity, for the analysis of a wider spectrum of target and for characterization of the two protein components apparently equipped with lytic activity.
|Number of pages||2|
|Publication status||Published - 2010|