Antifungal peptides produced by Bacillus amyloliquefaciens AG1 active against grapevine fungal pathogens

Santa Burruano, Giancarlo Moschetti, Antonio Alfonzo, Sandra Lo Piccolo, Valeria Ventorino, Gaetano Conigliaro

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Abstract Antifungal metabolites produced by Bacillus amyloliquefaciensAG1, previously isolated from wood of grapevinewith “esca syndrome”, were studied. The crude proteinextract (CPE) obtained from culture supernatant fluid byprecipitation with ammonium sulfate was assayed againstmany grapevine fungal pathogens. B. amyloliquefaciens strainAG1 showed a broad spectrum of antifungal activity, inhibitingmycelial growth in vitro of all tested fungal microorganisms.The metabolites contained in CPE were heat stable andremained active over a wide pH range (2–10). Their activitywas not affected by proteolytic or glycolytic enzymes. Tricine-SDS-polyacrylamide gel electrophoresis revealed a singleband within the range of 2,510–3,480 Da, that showed inhibitoryactivity when used in the antifungal assay. Mass spectrometryanalysis of this band allowed the substances involvedin antibiosis to be identified as two tryptic peptides thatcorrespond to the N-terminal sequence of subtilisin BPN’.These results suggest a potential role of B. amyloliquefaciensAG1 as a biocontrol agent.
Original languageEnglish
Pages (from-to)1593-1599
Number of pages7
JournalAnnals of Microbiology
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology

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