Analisi filogenetica condotta su ceppi di Bovine Diarrhea Virus (BVDV) isolati in Sicilia

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    Abstract

    [automatically translated] The Viral Diarrhea Cattle-Mucosal Disease, is an infectious disease that affects cattle, widespread worldwide. The infectious agent responsible is a virus envelope and provided with a single-stranded RNA genome with positive polarity and belongs to the genus Pestivirus, family Flaviviridae. Due to the ability to go against genetic mutations, there are a number of BVDV variants with different antigenic structure and different pathogenicity. To date two genotypes are known: BVDV-I and BVDV-II. The BVDV-I comprises at least 13 subtypes, while they were described only 2 for BVDV-II. The latter, not widespread in our area, is responsible for a highly lethal hemorrhagic syndrome. In Italy it was isolated from cattle that had been treated with anti-contaminated IBR vaccines. Recently it has also been hypothesized in Italy the presence of a third variant, the BVDV-III. The BVDV infection is often associated with disorders in the reproductive capacity and in pregnant cows may result in abortion, fetal malformations, or birth of calves persistently infected (PI). The purpose of this study was to genotype the BVDV isolates, in order to deepen the knowledge about the spread of several viral variants in Sicily. They were sequenced and phylogenetically analyzed 18 strains, isolated on MDBK cell lines, from samples of animals that were positive in RT-PCR specific for a portion of the region 5 'UTR and ELISA for viral antigen. For sequencing, viral strains were subjected to RNA extraction and amplification of a portion of the region 5 'UTR, as described by Vilcek et al. (1994). The obtained sequences were then aligned and compared with other available reference sequences in the GeneBank. Phylogenetic analysis conducted through the Neighbour-Joining method, has highlighted the presence of two viral subtypes belonging to the BVDV-I genotypes: BVDV-Ib and BVDV-Ie. No viral strain was found to belong to BVDV-II genotype. A further restriction of conduct analyzes on amplified using the restriction enzyme Avai, finally it confirmed the bovine origin of each isolate. The phylogenetic analysis of the viral strains isolated in a given territory,
    Original languageItalian
    Number of pages1
    Publication statusPublished - 2011

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