Analisi della comunità microbica intestinale di Reticulitermes lucifugus (Rossi) (Isoptera: Rhinotermitidae)

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Abstract

[automatically translated] The gut of termites hosts a microbial communities of bacteria and anaerobic flagellates, responsible for the degradation of lignocellulosic material. Digestion of lignocellulose by termites depends on the symbiosis with prokaryotes and eukaryotes microorganisms that live in their intestines. The micro-organisms convert the cellulose into sugars and acetate, producing hydrogen, methane and carbon dioxide. We have analyzed the intestinal microbial communities of Reticulitermes lucifugus lucifugus, one of two species of termites Italian, living in underground environments, it is destructive to woody structures of urban environments, causing serious damage to historic monuments and can colonize the homes, digging tunnels at ' internal support posts, beams and boards. Preliminary studies on the microbial community of symbiotic R. lucifugus, have described some species of protozoa (eg Dynenympha sp., Pyrsonimpha plagued Leidy, Spyrotriconimpha plagued Grassi and Foa, Holomastigotes elongatum, Triconympha agilis Leidy), but there are still no information on bacterial symbionts. Termite intestine was extracted metagenomic DNA, which has been amplified 16S rDNA PCR (polymerase chain reaction), using universal primers for prokaryotes. E 'it was built a library whose clones were grouped into OTU (Operational Taxonomic Units) using ARDRA (Amplified Ribosomal DNA Restriction Analysis analysis). For each OTU we were sequenced one or more clones and the sequences were compared with those present in the database. The sequence analysis showed that the microbial community of Reticulitermes lucifugus lucifugus is very diverse, there are clostridia, beta and delta proteobacteria, bacteria belonging to the genus Treponema and Uncultured Termite The group, present in the microbiota of other temiti addition to isolate the fraction cellulolytic bacteria from the gut of Reticulitermes lucifugus lucifugus, enrichment cultures were set up on different substrates, such as paper, eucalyptus, pine, walnut and CMC (carboxymethylcellulose). The isolates obtained were characterized and some positive results to the Congo red test identified. Preliminary results of this analysis show a prevalence of low GC gram positive bacteria belonging to the genus Bacillus, but also of alpha and beta proteobacteria.
Original languageItalian
Title of host publicationSistemi Biologici e Beni Culturali
Pages238-250
Number of pages13
Publication statusPublished - 2012

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