Abstract
The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).
| Original language | English |
|---|---|
| Pages (from-to) | 1116-1118 |
| Number of pages | 3 |
| Journal | Transplantation Proceedings |
| Volume | 41 |
| Publication status | Published - 2009 |
All Science Journal Classification (ASJC) codes
- Surgery
- Transplantation