TY - JOUR
T1 - ALCOHOLIC LIVER DISEASE: A MOUSE MODEL REVEALS PROTECTION BY LACTOBACILLUS FERMENTUM
AU - Tomasello, Giovanni
AU - Di Felice, Valentina
AU - Zummo, Giovanni
AU - Farina, Felicia
AU - Rappa, Francesca
AU - Barone, Rosario
AU - Cappello, Francesco
AU - Sangiorgi, Claudia
AU - Caruso Bavisotto, Celeste
AU - Marino Gammazza, Antonella
AU - Gammazza, Antonella Marino
AU - Macaluso, Filippo
AU - Barone, Rosario
AU - Cocchi, Massimo
AU - Rappa, Francesca
AU - Tomasello, Giovanni
AU - Bavisotto, Celeste Caruso
AU - De Macario, Everly Conway
AU - Cappello, Francesco
AU - De Macario, Everly Conway
AU - Macario, Alberto J.L.
AU - Di Felice, Valentina
PY - 2016
Y1 - 2016
N2 - Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.
AB - Objectives: Alcoholism is one of the most devasting diseases with high incidence, but knowledge of its pathology and treatment is still plagued with gaps mostly because of the inherent limitations of research with patients. we developed an animal model for studying liver histopathology, Hsp /heat-shock protein)-chaperones involvemnent, and response to treatment. Methods: The system was standardized using mice to which ethanol was orally administered alone or in combination with lactobacillus fermentum following a precise schedule over time and applying, at predetermined intervals, a battery of techniques (histology, immunohistochemistry, western blotting, real-time PCR, immunoprecipitation, 3-nitrosyne labelling) to assess liver pathology (e.g., steatosius, fibrosis), and Hsp60 and iNOS (inducible form of nitric oxide synthase) gene expression and protein levels, and post-translational modifications. Results: Typical ethanol-induced liuver pathology occurred and the effect of the probiotic coulkd be reliably monitored. steatosis score, iNOS levels, and nitrate proteins (e.g. Hsp60) decreased after probiotic intake. Conclusions: We describe a mouse model useful for studying liver disease induced by chronic ethanol intake and for testing pertinent therapeutic agents, e.g., probiotics. we tested L. fermentum which reduced considerably ethanol-induced tissue damage and deleterious post-translational modifications of the chaperone Hsp60. The model is available to test other agents and probiotics with therapeutic potential in alcoholic liver disease.
UR - http://hdl.handle.net/10447/168159
M3 - Article
VL - 7
JO - Clinical and Translational Gastroenterology
JF - Clinical and Translational Gastroenterology
SN - 2155-384X
ER -