Advanced fluorescence microscopy for in vivo imaging of neuronal activity

Giuseppe Sancataldo, And Francesco Saverio Pavone, Anna Letizia Allegra Mascaro, Ludovico Silvestri, Giuseppe Sancataldo, Leonardo Sacconi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Brain function emerges from the coordinated activity, over time, of large neuronal populations placed in different brain regions. Understanding the relationships of these specific areas and disentangling the contributions of individual neurons to overall function remain central goals for neuroscience. In this scenario, fluorescence microscopy has been proved as the tool of choice for in vivo recording of brain activity. Optical advances combined with genetically encoded indicators allow a large flexibility in terms of spatiotemporal resolution and field of view while keeping invasiveness in living animals to a minimum. Here we describe the latest advancements in the field of linear and nonlinear optical microscopy with special attention to the exploration of brain functionality of model animals. The present review aims to guide the reader through the main optical systems in the field toward future directions for in vivo microscopy applications.
Original languageEnglish
Pages (from-to)758-765
Number of pages8
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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