A Snapshot on MRSA epidemiology in a neonatal intensive care unit network, Palermo, Italy

Laura Saporito, Davide Vecchio, Daniela Maria Geraci, Caterina Mammina, Mario Giuffre, Celestino Bonura, Raffaele Pomo, Amalia Ciofalo, Giampiero Pinna, Angelo Rizzo, Vittoria Sepporta, Marcello Salvino Vitaliti, Giorgio Sulliotti, Fabio Lunetta, Aurora Aleo, Giorgio Graziano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014-January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A-F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.
Original languageEnglish
Pages (from-to)815-
Number of pages7
JournalFrontiers in Microbiology
Volume7
Publication statusPublished - 2016

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Neonatal Intensive Care Units
Methicillin-Resistant Staphylococcus aureus
Italy
Epidemiology
Nose
Multilocus Sequence Typing
Patient Transfer
Minisatellite Repeats
Hospital Records
Infection Control
Teaching Hospitals
Genes
Referral and Consultation
Prospective Studies
Anti-Bacterial Agents
Delivery of Health Care
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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A Snapshot on MRSA epidemiology in a neonatal intensive care unit network, Palermo, Italy. / Saporito, Laura; Vecchio, Davide; Geraci, Daniela Maria; Mammina, Caterina; Giuffre, Mario; Bonura, Celestino; Pomo, Raffaele; Ciofalo, Amalia; Pinna, Giampiero; Rizzo, Angelo; Sepporta, Vittoria; Vitaliti, Marcello Salvino; Sulliotti, Giorgio; Lunetta, Fabio; Aleo, Aurora; Graziano, Giorgio.

In: Frontiers in Microbiology, Vol. 7, 2016, p. 815-.

Research output: Contribution to journalArticle

Saporito, L, Vecchio, D, Geraci, DM, Mammina, C, Giuffre, M, Bonura, C, Pomo, R, Ciofalo, A, Pinna, G, Rizzo, A, Sepporta, V, Vitaliti, MS, Sulliotti, G, Lunetta, F, Aleo, A & Graziano, G 2016, 'A Snapshot on MRSA epidemiology in a neonatal intensive care unit network, Palermo, Italy', Frontiers in Microbiology, vol. 7, pp. 815-.
Saporito, Laura ; Vecchio, Davide ; Geraci, Daniela Maria ; Mammina, Caterina ; Giuffre, Mario ; Bonura, Celestino ; Pomo, Raffaele ; Ciofalo, Amalia ; Pinna, Giampiero ; Rizzo, Angelo ; Sepporta, Vittoria ; Vitaliti, Marcello Salvino ; Sulliotti, Giorgio ; Lunetta, Fabio ; Aleo, Aurora ; Graziano, Giorgio. / A Snapshot on MRSA epidemiology in a neonatal intensive care unit network, Palermo, Italy. In: Frontiers in Microbiology. 2016 ; Vol. 7. pp. 815-.
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abstract = "Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014-January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A-F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.",
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author = "Laura Saporito and Davide Vecchio and Geraci, {Daniela Maria} and Caterina Mammina and Mario Giuffre and Celestino Bonura and Raffaele Pomo and Amalia Ciofalo and Giampiero Pinna and Angelo Rizzo and Vittoria Sepporta and Vitaliti, {Marcello Salvino} and Giorgio Sulliotti and Fabio Lunetta and Aurora Aleo and Giorgio Graziano",
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TY - JOUR

T1 - A Snapshot on MRSA epidemiology in a neonatal intensive care unit network, Palermo, Italy

AU - Saporito, Laura

AU - Vecchio, Davide

AU - Geraci, Daniela Maria

AU - Mammina, Caterina

AU - Giuffre, Mario

AU - Bonura, Celestino

AU - Pomo, Raffaele

AU - Ciofalo, Amalia

AU - Pinna, Giampiero

AU - Rizzo, Angelo

AU - Sepporta, Vittoria

AU - Vitaliti, Marcello Salvino

AU - Sulliotti, Giorgio

AU - Lunetta, Fabio

AU - Aleo, Aurora

AU - Graziano, Giorgio

PY - 2016

Y1 - 2016

N2 - Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014-January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A-F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.

AB - Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014-January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A-F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.

KW - Active surveillance

KW - MRSA

KW - Microbiology

KW - Microbiology (medical)

KW - Molecular typing

KW - NICu

KW - Network approach

UR - http://hdl.handle.net/10447/181100

UR - http://journal.frontiersin.org/article/10.3389/fmicb.2016.00815full

M3 - Article

VL - 7

SP - 815-

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -