A real-time PCR quantitative detection assay for Pseudomonas savastanoi pv. nerii in Nerium oleander

Patrizia Bella, Patrizia Bella, Matilde Tessitori, Grazia Licciardello, Vittoria Catara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A real-time PCR assay based on TaqMan chemistry was developed for the detection of Pseudomonas savastanoi pathovars that cause bacterial knot disease on different plant species. Primers and probe sequences were based on the iaaL gene coding for (indole-3-acetyl)-L-lysine synthetase and previously used in conventional PCR tests. Assay specificity was tested with an extended range of strains of P. savastanoi from eight hosts, with 13 other Pseudomonas spp., and with other microorganisms naturally occurring on or in oleander plants. A pure culture cell suspension was quantified over a seven log concentration range (108 to 102 cfu ml-1 ). Different protocols were developed for the detection and quantification of P. savastanoi pv. nerii from symptomatic and asymptomatic oleander plants. A 24-h bacterial enrichment step either on PVF-1 or OKA-M broth improved the sensitivity of the assay, making it suitable to screen planting material for latent infections.
Original languageEnglish
Pages (from-to)204-213
Number of pages10
JournalPhytopathologia Mediterranea
Volume47
Publication statusPublished - 2008

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

Cite this