A method for measuring mitochondrial mass and activity

Anna Maria Rinaldi, Giovanni Morici, Maria Agnello

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Mitochondria, responsible for the energy-generating process essential for the cell metabolism,differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs.Using fluorescent probes specific for mitochondria (Mitotracker Green and Orange), and Confocal LaserScanning Microscope, we elaborated a method to measure in vivo the mitochondrial mass and activity, insea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variationof mitochondrial distribution and an increase of activity after fertilization.
Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalCytotechnology
Volume56
Publication statusPublished - 2008
Externally publishedYes

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