5' and 3' RACE Method to Obtain Full-Length 5' and 3' Ends of Ciona robusta Macrophage Migration Inhibitory Factors Mif1 and Mif2 cDNA

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The 5' and 3' RACE is a method to obtain full-length 5' and 3' ends of cDNA using known cDNA sequences from expressed sequence tags (ESTs), subtracted cDNA, differential display, or library screening. Here is described the identification of full-length 5' and 3' ends of Ciona robusta Mif1 and Mif2 cDNA by using 5' and 3' RACE method
Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Pages223-235
Number of pages13
Publication statusPublished - 2020

Publication series

NameMETHODS IN MOLECULAR BIOLOGY

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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